Hypothesis If the rate of oxygen consumption varies with it's unknown variable then the unknown variables will indicate it's rise in temperature. Materials 3 Cork pipetsAbsorbent cotton 3 Vials KOH Solution Laptop (Internet Access) Plastic Beads Germinated seeds (25) Non Germinated seeds (25) Timer Notebook 100 mL Graduated Cylinder Thermometer Tray Ice Silicone Glue Procedures Prior to Experiment... 1. Thoroughly read through the protocol 2. Predict future results of your experiment 3. Record observations Throughout Experiment 1. In a tray set up a room temperature bath (24 degrees Celsius) 2. In the second tray set up an ice bath (10 degrees Celsius) 3. Cont. to add ice in order to maintain 10 degrees Celsius 4. Label 6 vials "A, B, C, D, E, F" 5. Place a circle of absorbent cotton (about the size of a nickel) into the bottom of each vial 6. Soak cotton w/ 1-2 ml of the KOH solution (Must not allow any of the solution to touch the sides of the vials) 7. Place a circle of nonabsorbent cotton (about the size of a nickel) directly on top of the KOH soaked cotton circle in the vial. This will make sure no KOH touches the beans throughout the experiment 8. Fill the 100 ML graduated cylinder with 50 ml of water 9. Then add 25 germinated beans 10. Measure the increase in water volume. 11. Begin to record data into notebook 12. Place beans on paper towel (leave soaked in a paper towel GERMINATED BEANS ONLY) These will later be used in Vial A. 13. Repeat steps 5-8 for Vial D. 14. Fill the 100 ML graduated cylinder with 50 ml of water 15. Then add the 25 non-germinating beans 16. Add plastic beads in order to raise the volume of the water to be equal with the germinated beans 17. Remove the beans and beads and place them on a paper towel. These will later be used in Vial B. 18. Repeats steps for Vial E. 19. Fill the 100 ML graduated cylinder with 50 ml of water 20. Add plastic beads to raise the volume of the water to be equal with the germinated beans 21. Place beads on paper towel (dry the NON-GERMINATED BEANS) to be used in Vial C. 22. Repeat steps 16-18 for Vial F. 23. Place one set of germinating beans into Vial A. Seal with the cork/pipet assembly. 24. Repeat the previous step for Vial D. 25. Place one set of non-germinated beans and beads into Vial B. Seal with the cork/pipet assembly. 26. Repeat the previous step for Vial E. 27. Place one set of glass beads into Vial C. Seal with the cork/pipet assembly. 28. Repeat the previous step for Vial F. 29. Wrap Parafilm or plastic wrap tightly around the cork and tube seams. This will seal any potential leaks. 30. Check and make sure ice water bath is still 10 degrees Celsius. Record temperature of the room temperature bath. 31. Place strip of masking tap over water baths in order to prevent the pipets to decrease rate in temperature 32. Place Vials A, B, and C into ice water bath. Place them with the calibrated side of the pipet facing up. 33. Allow the respirometers to equilibriate for 8 minutes.
34. After it has reached the equilibration phase immediately submerge each respirometer. If any leaks are to be discovered in the vial resemble the faulty respirometer. (Recored any source of error) 35. Begin to record its temperatures. Continue to add ice or water as necessary to maintain that temperature. 36. Record the volume of the water in the pipet of every vial every 5 min for 20 min. 37. Correct volumes measured for changes in environmental variables. |